Ihor Vypasniak1, Sergii Popel1, Bohdan Mytskan1, Zınovii Ostapiak1, Iryna Ivanyshyn1
1Vasyl Stefanyk Precarpathian National University, Department of Physical Education and Sport, Ivano-Frankivsk, Ukraine
Reactive Changes of Buccal Epitheliocytes and Erythrocytes in Students with Different Somatic Health and Cardiorespiratory Endurance Levels
Sport Mont 2020, 18(2), 9-14 | DOI: 10.26773/smj.200620
Abstract
This paper aims to identify the specific features in buccal epitheliocytes’ and peripheral blood erythrocytes’ structural changes for students with different somatic health and cardiorespiratory endurance levels. Forty male students aged 17 were enrolled in this study, divided into three groups: 15 students with above-average and average somatic health levels, 15 students with below-average somatic health level, and a control group consisting of 10 healthy students. Cardio-respiratory endurance was determined using a 12-minute shuttle run test. Histological specimens were stained with azure and eosin and examined with a Leika DME light microscope. To obtain cell images, an Olympus Camedia C-480 ZOOM digital video camera was used. Determination of nucleus and cytoplasm structural perimeters on cytological medicine was performed using BioVision 4.0 software. Students with different somatic health levels were found to have regular reactive changes in the morphometric parameters of the buccal epitheliocytes and peripheral blood erythrocytes after cardiorespiratory endurance testing. As the proportion of discocytes decreases, and the contents of spherocytes and stomatocytes increases, a significant enrichment of microrelief and an increase of peripheral blood erythrocytes atypical forms occur, which indicates the low membrane resistance to the maximal aerobic physical activity in students with low somatic health. The revealed morphometric changes of buccal epitheliocytes and peripheral blood erythrocytes during physical activity are conditioned by the somatic health level, which affects the deformation degree of erythrocytes, limits the production of new, functionally active cells, and leads to a delay in the differentiation processes of cells within an increasing number of cells with raised core sizes, which closely correlates with the number of levels achieved during 12-minute shuttle run test.
Keywords
epitheliocytes, erythrocytes, morphometry, core-cytoplasmic ratio, somatic health, cardio-respiratory endurance
View full article
(PDF – 315KB)
References
Apanasenko, G., Volhina, L.N., & Bushuiev, Yu.V. (2000). Express screening of somatic health level of children and people of all ages: a methodical recommendations P. L. Shupik: Kiev.
Arutyunov, S.D., Pleskanovskaya, N.V., & Naumov, A.V. (2009). Periodontal diseases and “systemic diseases”: a known past, a promising future. Periodontology, 1, 3-6.
Baskevych, O.V., Popel, S.L., Gass, Yu.R., & Sobietov, B.H. (2017). Typical disorders of the organization of the membrane of erythrocytes during physical activity of different intensity in the process of development of the general endurance of the organism. Actual problems of modern medicine. Bulletin of the Ukrainian Medical Dental Academy, 2 (58), 8-14.
Belykh, E., Eskov, V., & Fudin, N. (2015). Exercise stress – fitness athlete criteria. J. New Medical Technologies, 9 (4), 34-40. https://doi.org/10.12737/17090.
Bulgakova, O.S. (2006). Mechanisms of change in the composition of peripheral blood and control of the dynamics of treatment by the method of registration of a common clinical blood test. Paper presented at the City scientific and practical conference “Topical issues of rehabilitation” (125-127), Saint Petersburg, Russia.
By’kov, K.M., Kargaev, P.P., & Frolov, B.S. (2012). Assessment of a person’s mental and mental instability by heart rate. Medical control equipment and methods. Leningrad.
Dranik, G.N. (2006). Clinical immunology and allergology (3rd ed., suppl.). Kiev: Polygraph Plus; il.
Duma, Z.V., Popel’, S.L., & Polyanskaya, N.I. (2016). Cellular reactions and the antioxidant protection system in long-term hypokinesia. Modern health-saving technologies, 4 (3), 131-140.
Faichak, R.I., Popel, S.L., & Sluchyk, V.M. (2011). Cytological effects of physical activity in students with different levels of training. Newsletter of Precarpathian University. Physical culture, 14, 23-28.
Gan, R.Z., & Popel, S.L. (2017). Morphological and biochemical mechanisms of changing buccal epithelocytes and erythrocytes in psycho-emotional stress. Regulatory Mechanisms in Biosystems, 8 (3), 363–368. https://doi.org/10.15421/021756.
Gasyuk, N.V. (2009). Morphofunctional organization of the gums in normal and inflammatory conditions: author. diss. … cand. med. sciences: 14.03.09. Simferopol.
Gol’dberg, E.D., Dy’gay, A.M., & Khlusov, I.A. (1997). The role of the autonomic nervous system in the regulation of hematopoiesis. Tomsk: Publishing House of Tomsk University.
Hande, A.H., & Chaudhary, M.S. (2010). Cytomorphometric analysis of buccal mucosa of tobacco chewers. Roman. J. of Morph. Embryol. 51 (3), 527-532.
Hoffman, R., Benz, E.J., Silberstein, L.E., et al. (2018). Hematology (Seventh Edition): Elsevier. https://doi.org/10.1016/B978-0-323-35762-3.00165-7.
Horizontov, P.D., Belousova, O.I., & Fedotova, M.I. (2013). Stress and blood system. M.: Medicine.
Korneva, E.A. (2015). Immunophysiology. SPb: Nauka.
Leshchak, O.M., & Popel, S.L. (2012). Reaction of buccal epithelium to standard physical activity in children of 8–10 years, who are in children’s health institution. Newsletter of Precarpathian University. Physical culture, 15, 44-52.
Levytskyi, V.A. (2007). Cytogenetic markers of somatic health of schoolchildren. Ukrainian medical almanac, 10 (3-Annex), 50-56.
Moshkin, M.P., & Gerlinskaya, L.A. (2016). Energetics, stress, immunity – a review of the development of population physiology. Zoological Journal, 95 (10), 1120-1135. https://doi.org/10.7868/s0044513416100056.
Muruzyuk, N.N., & Romanova, Yu.V. (2001). Normal indicators of the immunological status of an incoming population of working age in Nadym. Health Yamal, 6 (1), 26-29.
Nedospasov, S.A. (2013). Congenital immunity and its importance for biology and medicine. Bulletin of the Russian Academy of Sciences, 83 (9), 771-780. https://doi.org/10.7868/s0869587313090132.
Popel, S.L., & Mytskan, B.M. (2016). State of buccal epitheliocytes and erythrocytes and antioxidant protection system in long-term hypokinesia. Scientific journal of M.P. Dragomanov National Pedagogical University. Series 15. “Scientific and Pedagogical Problems of Physical Culture/Physical Culture and Sports”, 8 (78 K), 60-66.
Popel, S.L., Mytskan, B.M., & Lapkovskyi, E.Yo. (2017). Mechanism of changing adaptation potential and morpho-biochemical parameters of erythrocytes in students with different mode of day after physical load. Regulatory Mechanisms in Biosystems, 8 (2), 124-134. https://doi.org/10.15421/021711.
Prasad, H., Ramesh, V., & Balamurali, P.D. (2010). Morphologic and cytomorphometric analysis of exfoliated buccal mucosal cells in diabetes patients. J. Cytol., 27 (4), 113-117.
Pseunok, A.A., Dokhuzheva, F.Yu., & Mugotlev, F.Yu. (2000). Adaptation of the cardiovascular system in children. Valeology, 2, 48.
Rabinovich, O.F., Ostrovskij, A.D., & Abramova, E.S. (2013). Evaluation of mucosal immunity in students with dysbiosis of the oral mucosa before and after complex treatment. Immunology, 8, 91-94.
Raushenbakh, M.O., Zharova, E.I., & Khokhlova, M.P. (2012). Changing blood formula in stress. Arch. patol., 3, 23.
Sel’e, G. (1977). The concept of stress as we present it. New about hormones and their mechanisms of action. Kiev: Naukova dumka.
Vasil’ev, N.V., Zakharov, Yu.M., & Kolyada, T.I. (1992). Blood system and nonspecific resistance in extreme conditions. Novosibirsk: VO Nauka.
Vasilevskij, N.N., Sidorov, Yu.A., & Suvorov, N.B. (2013). On the role of biorhythmic processes in mechanisms of adaptation and correction of regulatory dysfunctions. Human physiology, 19 (1), 91-98.
Venkataswamy-Reddy, M. (2019). Chapter 19: Multivariate Statistical Methods. Chapter taken from Statistical Methods in Psychiatry Research and SPSS ISBN: 9780429023309 New York: Apple Academic Press (197-208). https://doi.org/10.1201/9780429023309-15.
Vejn, A.M., Solov’eva, A.D., & Kolosova, O.A. (1981). Vegetative-vascular dystonia. M.: Medicine.
Verkhoglyadova, L.M., Kurganova, L.V., Kachur, A.Yu., Mironova, N.I., & Pul’nyashenko, P.R. (2005). Interpretation of hematological examinations. Veterinary Hospital “Fauna – Service”, 1, 35-40.
Volchegorskij, I.A., Dolgushin, I.I., & Kolesnikov, O.L. (1998). The role of the immune system in the organism adaptation strategy selection. Cheljabinsk: Nauka.
Wandeur, T., de Moura, S.A., & de Medeiros, A.M., et al. (2011). Exfoliative cytology of oral mucosa in burning mouth syndrome: a cytomorphological and cytomorphometric analysis. Gerodontology, 28 (1), 44-48.
Zemska, N.O., & Piatnychuk, G.O. (2017). Functional systems of students’ organism depending on the lev-el of physical fitness for physical activity. Physical Education of Students, 6, 25-30.